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Can CRISPR-Cas gene editing tech fight antimicrobial resistance?

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New Delhi, April 28 (IANS) CRISPR-Cas, the Nobel Prize-winning gene-editing technology, has the potential to target resistance genes and make bacteria sensitive to first-line antibiotics again, according to a study.

CRISPR-Cas, which functions like molecular “scissors”, allows for precise alterations to the genomes of living organisms. This revolutionary technique, which brought its inventors, Jennifer Doudna and Emmanuelle Charpentier, the Nobel Prize in Chemistry in 2020, can cut the DNA at designated spots — deleting unwanted genes or introducing new genetic material into an organism’s cells, paving the way for advanced therapies.

Even as antimicrobial resistance (AMR) is continuing to increase globally, researchers from the University of Copenhagen noted that CRISPR/Cas systems can also be employed for developing antimicrobial agents. It can effectively and selectively kill target bacterial populations, they noted in the study, presented at the ongoing ESCMID Global Congress in Barcelona.

Dr Rodrigo Ibarra-Chavez, Department of Biology, University of Copenhagen, said that CRISPR-Cas systems (a bacterial immunity system) work by inducing “bacterial cell death or interfering with antibiotic resistance expression”.

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However, bacteria have ways to fight back as they also possess built-in anti-CRISPR systems that can repair any damage caused by CRISPR-Cas systems. Their research proposes creating guided systems against AMR genes that could treat infections and prevent the dissemination of resistance genes via mobile genetic elements (MGEs).

MGEs are parts of the bacterial genome that can move to other host cells or also transfer to another species.

Dr Ibarra-Chavez noted that repurposing MGEs and choosing the delivery mechanism involved in the antimicrobial strategy is important for reaching the target bacterium. The method removes bacteria’s resistance power and makes “them vulnerable again to first-line antibiotics”, the study said.

–IANS

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As Maha detects 91 FLiRT Covid cases, expert rules out any immediate concern

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New Delhi, May 12 (IANS) Even as the 91 cases of new FLiRT Covid-19 variants, accounting for more than a third of cases in the US, have reportedly been detected in Maharashtra, there is nothing to worry immediately, a top expert said on Sunday.

The new set of variants dubbed FLiRT, majorly includes KP.1.1, and KP.2 strains. These are named based on the technical names for their mutations, one of which includes the letters “F” and “L”, and another of which includes the letters “R” and “T”.

Genome sequencing of March and April showed that Maharashtra has 91 cases of KP.2 — Pune (51), Thane (20), Amravati (7) Aurangabad (7), Solapur (2), Ahmednagar (1), Nashik (1), Latur (1), and Sangli (1).

“What we see now, is the result of genome sequencing of the last two months, that is March and April. So, there is no cause of concern immediately as out of the 91 cases, not a single death, hospitalisation, or severe disease has been reported,” infectious disease expert Dr Ishwar Gilada, told IANS.

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“Even the test positivity rate is around 1 per cent,” he added.

Dr Rajesh Karyakarte, Maharashtra’s genome sequencing coordinator, said that KP.2 has become the predominant Covid strain in the state.

However, there hasn’t been a corresponding increase in hospitalisations or severe cases, The Times of India reported.

FLiRT variants come from the lineage of the highly transmissible and immune system-evading Omicron.

First identified globally in January, KP.2 is a descendant of Omicron’s JN.1. According to the data from the US Centers for Disease Control and Prevention, KP.2 accounted for about one in four or 25 per cent of new sequenced cases in the country in the last weeks of April.

Similar to previous variants, the symptoms typically include a sore throat, runny nose, congestion, tiredness, fever (with or without chills), headache, muscle pain, and sometimes loss of taste or smell.

“Covid has not gone away, but it is not creating any havoc. It is not creating any hospitalisation or deaths, and it is much milder than the flu. So, it should not be considered a separate disease. It should be considered like the flu and we can call it Covi-flu,” said Dr Gilada, Consultant in Infectious Diseases Unison Medicare and Research Centre, Mumbai.

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However, he called for proper whole genome sequencing.

“Though the variant will become prominent in numbers, it is not causing a surge in demand for oxygen, beds, ICUs, or ventilators, and there are also no deaths. “So, I think we should not be worried and concentrate on other emerging health problems,” he said.

–IANS

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'Anaemia a preventable health threat in girls, women in India'

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New Delhi, May 12 (IANS) Anaemia is a very common yet preventable threat among girls and women in India, said health experts on Sunday.

A deficiency of healthy red blood cells or haemoglobin needed to carry oxygen to the body’s tissues raises the risk of anaemia. The condition is more common among women and girls. Lack of a proper iron-rich diet, particularly among women in their reproductive age can result in tiredness, weakness, and shortness of breath, the experts noted.

According to the National Family Health Survey 5 (NFHS-5, 2019-21), 25 per cent of men (aged 15-49 years) and 57 per cent of women (aged 15-49 years) suffer from anaemia.

“Anaemia is a very common and another rampant problem in Indian ladies, especially, due to intake of a diet low in iron and sometimes, it is genetically determined also. A vegetarian diet is quite low in iron and hence, supplements are necessary,” Dr M. Wali, Senior Consultant, Department of Medicine, Sir Ganga Ram Hospital told IANS.

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“Pregnancy increases the requirement of iron and also lactation, so these two conditions are particular to the females,” he added.

The doctor further noted that anaemia is prevalent in both rural and urban women in India.

“Most Indian urban women who are dieting to reduce weight become deficient and rural women because of hard work, increased requirements, increased loss of the menstrual blood due to infection or otherwise repeated childbirth, so all these causes lead to lack of blood or anaemia,” Dr Wali said.

The NFHS- data shows that anaemia is also highly prevalent among young adolescent boys (31 per cent), and adolescent girls (59 per cent) aged between 15 and 19 years, as well as in pregnant women (52.2 per cent) between 15 and 49 years, and also in small children (67 per cent) aged between 6 months and below 5 years.

Common indicators of anaemia include fatigue, pale complexion, breathlessness, dizziness, and cold extremities.

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“Anaemia poses a significant health challenge in India, especially for women as they are more prone to lower haemoglobin levels owing to their distinct physiological requirements, including menstrual and pregnancy-related blood loss,” Dr Rahul Bhargava, Principal Director & Chief BMT, Fortis Memorial Research Institute, Gurugram, told IANS.

Importantly, the health experts pointed out that the condition progresses slowly and discreetly, and is difficult to detect. The symptoms also tend to appear late. They advised eating foods rich in iron or taking supplements.

“Anaemia is very difficult to detect, most of the time it is slowly developing. Symptoms like breathlessness (an important symptom), chest pain (sometimes mistaken as heart pain), difficulty in pregnancy, palpitation, headache, and easy fatigability appear very late,” Dr Wali said.

“Many women quietly battle anaemia — a preventable threat in India. By promoting a balanced diet rich in iron, advocating for accessible iron supplements available through government systems, and emphasising regular screening, we can empower women to combat anaemia and embrace healthier lives together,” Dr Ameet Babre, National Programme Manager at Nutrition International, told IANS.

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The most effective preventive measures against anaemia involve adopting an iron-rich diet, which includes lean meats, poultry, fish, legumes, tofu, leafy greens like spinach and kale, fortified cereals, and nuts, Dr Bhargava said.

Fruits like guava, banana, figs, and pomegranate may also help combat anaemia.

–IANS

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89 pc Indian mothers concerned about kids' screen time

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New Delhi, May 12 (IANS) A whopping 89 per cent of Indian mothers are concerned about the screen time of their children, according to a report on Sunday.

The report, released by market research firm Techarc on Mother’s Day, is based on a survey of 600 working mothers, across four metro cities, having at least one child between grade 3-10 in school.

It explores insights on the digital ecosystem in which mothers operate today viz-a-viz the challenges, concerns, interests, and preferences.

“Mothers believe that increased screen time affects their studies and has a negative impact on the mental and physical health of their children,” the report said.

Further, the report showed that privacy (81 per cent), inappropriate content (72 per cent), teenage influencers (45 per cent), deep fakes, and impersonation (26 per cent) were other top concerns bothering mothers.

Mothers feel that deep fakes and Gen AI will add to their future parenting worries as they get to see the rising cases along with learning how these can be negatively used to malign the reputation and credibility of individuals.

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Among the devices, mothers are concerned about the expected popularity of VR headsets, especially after the launch of Apple Vision Pro.

Mothers believe that “this form factor will zone out their children who will lose attentiveness towards the environment adding to their distractions,” the report said.

On the other hand, mothers also found the digital world much more useful and relevant for children, compared to five years ago.

More than 60 per cent of the mothers spent 51-85 per cent on their children through digital mediums buying various goods and services. Nearly 20 per cent of digital savvy mothers spent more than 85 per cent, the report said.

Mothers’ most trusted digital partners include Amazon (for shopping), Swiggy (for food), and Disney Hotstar (for entertainment)

–IANS

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More than 1 in 5 new mothers experience postpartum depression: Doctors

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New Delhi, May 12 (IANS) While parenthood comes as a blessing for many, for more than 20 per cent of mothers, the new chapter of life creates stress, anxiety and postpartum depression, which can turn fatal for both the mother and child, without adequate support, said doctors on Mother’s Day on Sunday.

Mother’s Day is celebrated every year on the second Sunday of May.

Postpartum depression is common but a treatable medical condition that many women face after childbirth. While the exact cause can be difficult to identify, several factors contribute to these feelings of sadness, anxiety, and tiredness.

These can result from genetics, hormonal changes, sleep deprivation, fatigue, or the pressure of becoming a mother.

The overall pooled estimate of the prevalence of postpartum depression was 22 per cent reporting postpartum depression within two weeks of delivery.

“The journey to parenthood presents couples with a myriad of challenges, often impacting their emotional well-being profoundly. The complexities surrounding late pregnancies, assisted conception methods like IVF, and the burden of preterm deliveries weigh heavily on maternal mental health,” Dr Saurabh Mehrotra, Associate Director, Psychiatry, Institute of Neurosciences, Medanta, Gurugram, told IANS.

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Studies reveal that maternal mental illness during pregnancy correlates with adverse outcomes for both mother and child, including preterm delivery and poor neurodevelopment.

“At Medanta, we witness approximately 70-80 per cent of mothers having post-partum blues out of which 20 per cent of mothers with postpartum depression grapple with such mental health challenges, emphasising the critical need for comprehensive emotional support and holistic care throughout the perinatal period,” Dr Saurabh said.

The symptoms of postpartum depression include insomnia, loss of appetite, intense irritability, and even difficulty bonding with the baby. For mothers facing the postpartum blues, asking for help may be one of the most crucial steps, as it may help them easily bond with the baby, the experts said.

But, “if left untreated, the condition can last several months or longer,” Dr Teji Dawane, Senior Consultant, Obstetrician and Gynaecologist, Motherhood Hospitals, Whitefield, Bengaluru, told IANS.

“One of the first steps in addressing postpartum depression is early detection through screenings during prenatal and postnatal care and giving importance to emotional well-being. It is also encouraged to seek help from professional support services such as counselling and therapy,” Dr Teji said.

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Sometimes, treatment options also include medication like antidepressants to manage symptoms effectively. It is equally important to create a supportive family environment and develop self-care practices for new parents, the experts noted.

–IANS

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Premature menopause may raise risk of early death: Study

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New Delhi, May 12 (IANS) Women who enter menopause before the age of 40 are more likely to die young, finds a study.

The study presented at the 26th European Congress of Endocrinology in Sweden, however, showed that the risk can be lowered with hormone replacement therapy (HRT) — the most common treatment.

While most women experience menopause between the ages of 45 and 55, about 1 per cent experience menopause before the age of 40 years, known as premature menopause or premature ovarian insufficiency (POI). It increases the risk of long-term health problems such as heart disease.

The reason behind this remains largely unknown but can be brought on spontaneously or by some medical treatments such as chemotherapy or by surgically removing the ovaries.

The team from the University of Oulu, in Finland, examined 5,817 women who were diagnosed with spontaneous or surgical premature ovarian insufficiency in Finland, between 1988 and 2017, and compared them with 22,859 women without POI.

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The results revealed spontaneous premature ovarian insufficiency more than twice raised the risk of dying of any cause or heart disease, and more than four times from cancer.

On the other hand, the risk of all-cause and cancer mortality halved in women who used HRT drugs for more than six months. Further, women with early menopause from surgery were not found to have any added mortality risk.

“Our findings suggest specific attention should be paid to the health of women with spontaneous premature ovarian insufficiency to decrease excess mortality,” said Hilla Haapakoski, a doctoral student at the University of Oulu, in Finland.

–IANS

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